recombinant gro α  (R&D Systems)

Journal: PLOS ONE

Article Title: Anti-necroptotic effects of human Wharton’s jelly-derived mesenchymal stem cells in skeletal muscle cell death model via secretion of GRO-α

doi: 10.1371/journal.pone.0313693

Figure Lengend Snippet: Proteins secreted from the medium were analyzed using a RayBio Biotin Label-based Human Antibody Array. (A) Increased spot intensity for GRO-α was observed in cells derived from the same donor. The selection of intersecting proteins among candidate proteins secreted by hWJ-MSCs was higher than that of hPL-MSCs. (B) The conditioned media was collected from C2C12, hWJ-MSCs alone, hPL-MSCs alone, and co-cultured cells. The concentration of secreted GRO-α in each conditioned medium was measured using the GRO-α ELISA kit (** p < 0.01, * p < 0.05, n = 4). Data are significantly different from that in the corresponding control group.

Article Snippet: The Human GRO-α Quantikine ELISA kit (#DGR00B; R&D systems, USA) was used to analyze the expression of proteins selected from the antibody array.

Techniques: Ab Array, Derivative Assay, Selection, Cell Culture, Concentration Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: PLOS ONE

Article Title: Anti-necroptotic effects of human Wharton’s jelly-derived mesenchymal stem cells in skeletal muscle cell death model via secretion of GRO-α

doi: 10.1371/journal.pone.0313693

Figure Lengend Snippet: In vitro damaged C2C12 cells were co-cultured with hWJ-MSCs pretreated with GRO-α siRNAs, which reduced the levels of secreted GRO-α. (A) To examine knockdown of GRO-α in hWJ-MSCs, the medium was collected and GRO-α levels were analyzed using ELISA (*** p <0.001, n = 3). (B) Harvested cells were analyzed through western blotting with specific antibodies (p-RIP3, p-MLKL, β-actin). (C, D) All bands were analyzed through densitometry (** p <0.01, * p < 0.05, n = 3).

Article Snippet: The Human GRO-α Quantikine ELISA kit (#DGR00B; R&D systems, USA) was used to analyze the expression of proteins selected from the antibody array.

Techniques: In Vitro, Cell Culture, Knockdown, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: NPJ Precision Oncology

Article Title: A patient-designed tissue-engineered model of the infiltrative glioblastoma microenvironment

doi: 10.1038/s41698-022-00290-8

Figure Lengend Snippet: a Cytokine expression based on a Luminex array for hydrogel samples of astrocytes and microglia alone (AM) and in combination with GSC lines G2, G34, or G528 in static conditions. The baselines for each glia-only and GSC monoculture condition are subtracted from the respective tri-culture condition to show synergistic as opposed to additive effects. Therefore, the total height of each bar (as opposed to relative height) shows the total cytokine expression for each condition. b Schematic showing what is known about the three cytokines pertaining to glial expression following activation and the known effects on glioma invasion, proliferation, and stemness (counterclockwise from left). c – e Effects of blocking the cytokine receptors CXCR2 (for CXCL1 and CXCL8) and CCR2 (for CCL2) in tri-culture under flow conditions on the outcomes of invasion ( c ), proliferation ( d ), and stemness ( e ). f – h Effects of adding CCL2 to GSC monoculture hydrogels vs. the full cellular TME in static conditions for invasion ( f ), proliferation ( g ), and stemness ( h ). Statistics performed by paired t -tests with * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Neutralizing antibodies included 0.2 μg/mL α-CXCL8 (R&D Systems MAB208), 2 μg/mL α-CCL2 (R&D Systems MAB279), and 7 μg/mL α-CXCL1 (R&D Systems MAB275).

Techniques: Expressing, Luminex, Activation Assay, Blocking Assay